Confocal Microscopes

Nikon Eclipse Ti-E Inverted Fluorescence Microscope (for fixed cells, live-cell imaging & high-throughput imaging)

  • A1R laser scanning confocal system
  • LU-N4/N4S 4-laser unit (405 nm, 488 nm, 561 nm, 640 nm)
  • Ultrafast resonant scanner (Up to 512 x 32 pixels at 420 fps)
  • High-resolution galvano scanner (Up to 4096 x 4096 pixels)
  • A1-DUG-2 GaAsP confocal multi-detector unit
  • A1-DUS spectral detector unit (Up to 32-channel spectral image at 24 fps)
  • Perfect focus system for live-cell imaging
  • Lumencor SPECTRA X independent light engine (395, 440, 470, 510, 550, 575, 640 nm)
  • Andor Zyla 4.2 PLUS sCMOS camera
  • Tokai INU microscope incubator with temperature controller for top, stage, bath and lens and in-line 5% CO2 (for live-cell imaging)
  • NIS Elements – High Content Analysis (HC) software

Nikon Eclipse FN1 Upright Fluorescence Microscope (for optogenetics, brain slices & tissue)

  • A1R multiphoton laser scanning confocal system
  • Coherent Chameleon II Vision multiphoton laser unit
  • Episcopic GaAsP NDD multiphoton detector unit
  • A1-DUS spectral detector unit
  • LU-N4/N4 4-laser unit (405 nm, 488 nm, 561 nm, 640 nm)
  • Ultrafast resonant scanner (Up to 512 x 32 pixels at 420 fps)
  • High-resolution galvano scanner (Up to 4096 x 4096 pixels)
  • A1-DUG-2 GaAsP confocal multi-detector unit
  • High-speed piezo objective-positioning system
  • DS-Fi1c high definition cooled color digital camera
  • Lumencor SOLA SE white light engine
  • CFI 75 Apochromat multiphoton objective with 25X, water immersion, NA 1.10, WD 2.00 mm, correction ring, and anti-reflective coating
  • NIS Elements – Confocal (C) software

Nikon Eclipse Ti-E Inverted Fluorescence Microscope (at UPR-Institute of Neurobiology)

  • A1R laser scanning confocal system
  • LU-N4/N4S 4-laser unit (405 nm, 488 nm, 561 nm, 640 nm)
  • Ultrafast resonant scanner (Up to 512 x 32 pixels at 420 fps)
  • High-resolution galvano scanner (Up to 4096 x 4096 pixels)
  • A1-DUG-2 GaAsP multi-detector unit
  • A1-DUS spectral detector unit (Up to 32-channel spectral image at 24 fps)
  • Perfect focus system for live-cell imaging
  • Lumencor SOLA SE white light engine (395 nm, 420-680 nm)
  • NIS Elements – Confocal (C) software

ELECTROPHYSIOLOGY RIGS

Patch Clamp Rig (for brain slices and tissue)

  • Coupled to Nikon A1R multiphoton FN1 upright fluorescence microscope
  • Axopatch 200B amplifier
  • Axon Digidata 1550B digitizer
  • P.I. Master-9 pulse stimulator
  • Sutter MPC-200 multi-micromanipulator system
  • Axon pCLAMP 10 software

Patch Clamp Rig (for isolated cells & cell lines)

  • Coupled to Nikon Ti2-A inverted fluorescence microscope
  • HEKA 10 USB amplifier
  • Andor Zyla 4.2 PLUS sCMOS camera
  • Thorlabs PCS-5200 micromanipulator
  • Warner TC-344C temperature controller
  • Warner SH-27B in-line solution heater
  • Warner QE-1 quick exchange chamber platform
  • Lumencor SOLA SE white light engine (395 nm, 420-680 nm)
  • HP Z4 G4 workstation computer
  • HP 24” Z24N G2 monitor
  • HEKA PatchMaster software
  • Andor iQ software

Patch Clamp Rig (for isolated cells & cell lines)

  • Coupled to Olympus IX70 inverted fluorescence microscope
  • Axopatch 200B amplifier
  • Axon Digidata 1440A digitizer
  • Sutter MP-285 micromanipulator
  • Warner VC-6 valve controller
  • Warner SF-77B fast stepper perfusion system
  • Warner TC-344C temperature controller
  • Warner SH-27B in-line solution heater
  • Warner QE-1 quick exchange chamber platform
  • Olympus BH2-RFL-T3 mercury arc lamp with 100 W bulb (lEx: 254, 297-302, 312-313, 334, 365, 405, 436, 546, and 579 nm)
  • Andor iXon EMCCD camera
  • Axon pCLAMP 10 software
  • Andor iQ software

Sharp Electrodes Intracellular Recording Rig (for tissue)

  • Coupled to Olympus BX51WI upright fluorescence microscope
  • Axoclamp 900A amplifier
  • Axon Digidata 1440A digitizer
  • Sutter MPC-200 multi-micromanipulator system
  • Sutter ROE-200 micromanipulator controller
  • Axon pCLAMP 10 software

Two-Electrode Voltage Clamp Rig (for frog oocytes)

  • GeneClamp 500 amplifier
  • Axon Digidata 1440A digitizer
  • Warner VC-8 valve controller
  • Axon pCLAMP 10 software

Two-Electrode Voltage Clamp Rig (for frog oocytes)

  • GeneClamp 500B amplifier
  • Axon Digidata 1440A digitizer
  • Warner VC-8 valve controller
  • Axon pCLAMP 10 software

BILAYER STATION

Planar Lipid Bilayer Workstation

  • Warner BC-535 bilayer clamp amplifier
  • Warner FC-1 Faraday cage with manual vibration isolation table
  • Warner LPF-8 Low-pass Bessel filter
  • Warner BPS-2 Bilayer Perfusion system
  • Warner SUNStir-3 Dual function controller for lamp stirplate
  • Axon pCLAMP 10 software

ELECTROMYOGRAPHY (EMG) STATION

Electromyography Workstation (for small animals)

  • AD PowerLab 4/30 system
  • AD ML408 Dual Bio amplifier and stimulator
  • AD LabChart 6 software

WORKSTATION COMPUTERS (for imaging analysis)

HP Z8 G4 workstation computer

  • Operating System: Windows 10 Pro 64-bit English
  • Chassis: HP Z8 G4 90 1125W Chassis 100V/15A
  • Processor: 2X Intel® Xeon® Gold 5122 Processor (3.6 GHz, up to 3.7 GHz w/Turbo Boost, 16.5 MB cache, 2666MHz, 4 core), (8 Total Cores)
  • Memory: 128 GB (8×16 GB) DDR4-2666 ECC Registered Memory (2 Processors)
  • Internal OS Load Storage Options: Operating System Load to M.2
  • Internal M.2 Storage: 512 GB HP Z Turbo Drive TLC M.2 SSD
  • 2nd Internal M.2 Storage: 1 TB HP Z Turbo Drive TLC M.2 2nd SSD
  • Internal Storage: 4X 2 TB 7200 RPM SATA 3.5” HDD, (8 TB Total)
  • Graphic Card: NVIDIA® Quadro® P4000 (8 GB GDDR5, 4X Displayport 1.4) Graphics
  • Networking: Intel® X550-T2 10GbE Dual Port NIC
  • Ports: Premium – 2X USB 3.1 Type C; 2 x USB 3.0 Type A
  • Keyboard: USB Premium Wired Keyboard
  • Mouse: HP Wired Optical USB Mouse
  • HP 24” Z24N G2 monitor
  • Nikon NIS Elements – Advance Research (AR) software
  • Nikon NIS Elements – Extended Depth of Focus (EDF) module
  • Nikon NIS Elements – Calcium Ratio and FRET module
  • Nikon NIS Elements – General Analysis in 2D and 3D module
  • Nikon NIS Elements – 2D and 3D Measurement Tracking module
  • Nikon NIS Elements – Laboratory Imaging (LIM) 2D and 3D Deconvolution module
  • Nikon NIS Elements – Additional Batch Offline LIM Deconvolution module

HP Z8 G4 workstation computer (at UPR-Institute of Neurobiology)

  • Operating System: Windows 10 Pro 64-bit English
  • Chassis: HP Z8 G4 90 1125W Chassis 100V/15A
  • Processor: 2X Intel® Xeon® Gold 5122 Processor (3.6 GHz, up to 3.7 GHz w/Turbo Boost, 16.5 MB cache, 2666MHz, 4 core), (8 Total Cores)
  • Memory: 128 GB (8×16 GB) DDR4-2666 ECC Registered Memory (2 Processors)
  • Internal OS Load Storage Options: Operating System Load to M.2
  • Internal M.2 Storage: 512 GB HP Z Turbo Drive TLC M.2 SSD
  • 2nd Internal M.2 Storage: 1 TB HP Z Turbo Drive TLC M.2 2nd SSD
  • Internal Storage: 4X 2 TB 7200 RPM SATA 3.5” HDD, (8 TB Total)
  • Graphic Card: NVIDIA® Quadro® P4000 (8 GB GDDR5, 4X Displayport 1.4) Graphics
  • Networking: Intel® X550-T2 10GbE Dual Port NIC
  • Ports: Premium – 2X USB 3.1 Type C; 2 x USB 3.0 Type A
  • Keyboard: USB Premium Wired Keyboard
  • Mouse: HP Wired Optical USB Mouse
  • HP 24” Z24N G2 monitor
  • Nikon NIS Elements – Advance Research (AR) software
  • Nikon NIS Elements – Extended Depth of Focus (EDF) module
  • Nikon NIS Elements – Calcium Ratio and FRET module
  • Nikon NIS Elements – General Analysis in 2D and 3D module
  • Nikon NIS Elements – 2D and 3D Measurement Tracking module
  • Nikon NIS Elements – Laboratory Imaging (LIM) 2D and 3D Deconvolution module
  • Nikon NIS Elements – Additional Batch Offline LIM Deconvolution module

WORKSTATION COMPUTERS (for imaging analysis)

DELL XPS 15 Laptop (2 laptops)

  • Operating System: Windows 10 Pro 64-bit English
  • Processor: 8th Generation Intel® Core™ i7-8750H Processor (9MB Cache, up to 4.1 GHz, 6 Cores)
  • Memory: 32GB 2x16GB DDR4-2666MHz,
  • Hard Drive: 1TB PCIe Solid State Drive
  • Video Card: NVIDIA® GeForce® GTX 1050Ti with 4GB GDDR5 graphics
  • Display: 15.6″ FHD 1920 x 1080 Anti-Glare Non-touch IPS 100% sRGB 400-Nits display InfinityEdge
  • Wireless: Killer 1535 802.11ac 2×2 WiFi and Bluetooth 4.2
  • Software: (TBA)

DELL XPS 15 Laptop (2 laptops at UPR-Institute of Neurobiology)

  • Operating System: Windows 10 Pro 64-bit English
  • Processor: 8th Generation Intel® Core™ i7-8750H Processor (9MB Cache, up to 4.1 GHz, 6 Cores)
  • Memory: 32GB 2x16GB DDR4-2666MHz,
  • Hard Drive: 1TB PCIe Solid State Drive
  • Video Card: NVIDIA® GeForce® GTX 1050Ti with 4GB GDDR5 graphics
  • Display: 15.6″ FHD 1920 x 1080 Anti-Glare Non-touch IPS 100% sRGB 400-Nits display InfinityEdge
  • Wireless: Killer 1535 802.11ac 2×2 WiFi and Bluetooth 4.2
  • Software: (TBA)